ABSTRACT Tillage is an important management tool for tackling and promoting water conservation and improving crop yield. As one of the important nutrients in plant growth, K is involved in important processes such as osmoregulation, photosynthesis and metabolite transport, and plays a particularly critical role in improving crop yield and quality. In the long-term positioning platform of the tillage method, a 2-yr field experiment was conducted in 2019-2020 in maize (Zea mays L.) Three tillage methods: conventional tillage (CT), subsoil tillage (ST), and no-tillage (NT) and two planting densities 6×104 (D1) and 9×104 plants ha-1 (D2) were set up in the experiment. The results showed that yield and K translocation efficiency (KTE) were significantly higher in NT than in CT at D1 (by 4.7% and 12.2%) and D2 (by 14.0% and 13.9%), respectively. At maturity stage in 2019, population DM accumulation after silking (DMA) was significantly higher in NT (by 11.0% and 16.9%) than in CT at D1 and D2. Correlation analysis revealed that yield was significantly positive correlated with ears (r = 0.57***) and DMA (r = 0.64***). Potassium translocation and K harvest index were positively correlated with KTE. Under spring drought year, the long-term no-tillage had a significant yield increase, mainly through the increase in 1000-kernel weight. The increase in K efficiency was mainly through the influence of DM accumulation and distribution, and K accumulation in grain.

Cordyceps is a fastidious pathogenic fungus infecting insects, and recent years have witnessed rapid progress in its medical properties. In this study, a wild isolate, C. cicadae MP12, was characterized through in vitro cultivation and its nuclear small-subunit (SSU) ribosomal DNA (rDNA) data. In vitro culture of C. cicadae MP12 was established by growing its fruiting bodies in a solid matrix. C. cicadae MP12 was inoculated into Cryptotympana atrata cicada pupae for in vivo culture, where the fungi developed its fruiting body as well. The contents of adenosine and cordycepin in dried fruiting bodies after culture were 1421.45µg/g and 1398.12 µg/g, respectively. Therefore, the established cultures from this study could be used for the production of various medically important metabolic substances.